Genotyping and primer details


Genotyping and primer details for SALK_042400 - At1g77330
Line ID SALK_042400
Locus At1g77330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29063055 of line SALK_042400 Chr1:29063055
   
Insertion allele amplicon R204 - BI34
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer BI34: 5'-CAACACTGGTGATCAGATTGAAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29063619
   
wt amplicon (over InsPos) BI35 - BI34
wt amplicon start Chr1:29062621
wt amplicon end Chr1:29063619
wt amplicon size 999 bp
Locus specific primer BI34: 5'-CAACACTGGTGATCAGATTGAAGT-3'
2nd locus specific primer BI35: 5'-AGAAATTTGCAGGTTGTTGGTTAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11