Genotyping and primer details

Genotyping and primer details for SALK_042514c - At5g05670
Line ID SALK_042514c
Locus At5g05670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1697189 of line SALK_042514c Chr5:1697189
Insertion allele amplicon LBb3 - 15ss
Locus specific primer 15ss: 5'-CTTCATTTGGTTCCATTGATGTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1696744
wt amplicon (over InsPos) 22tf - 15ss
wt amplicon start Chr5:1696744
wt amplicon end Chr5:1697425
wt amplicon size 682 bp
Locus specific primer 15ss: 5'-CTTCATTTGGTTCCATTGATGTTA-3'
2nd locus specific primer 22tf: 5'-TGTTGTTTACAACCATCTTGCTCT-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11