Sequences from confirmation amplicon


Confirmation sequences for line SALK_042514c and hit At5g05670 are shown.
Line ID SALK_042514c
Locus At5g05670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1697189
Primers used for PCR 15ss - LBb3, show primer details
   
Sequence Name 19-K116528-SALK_042514C-15ss
GenBank Accession LN484826 [GenBank]
Sequencing primer gene specific primer, 15ss: 5'-CTTCATTTGGTTCCATTGATGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K116528-SALK_042514C-15ss
ATCAAACAATTATCGTGTTATAACTCTCCACAATGACCTAGTCACTCACACTGACTCACA
ATAGTGACACATTACAAAAAAAAAGTACATTTGAAGGAAAGTCATAAACAATATTCATAT
GAAAAAGGTAACGTCAAGCCAAAACACTCACTTGGTAAAAGAGCACAGTCTTTCCACTTC
CGGTCAGCCCAGAAAGGAGTACAGTGTTGGATTTGGTACGTCTGACCAGGCGAACTGCAT
ACCCCAGCAAGAACAACAAGTTTAGCAACAAAAGATCAAAAGTTTGTTTATGTATAATCA
AAGAAAAATCATGAATAAACCTCAGAAATTGCAATAATAGTTCTAATTCCAATACACAAA
ATACTCTGGCTTAATCACAAGTCTGATCATGAGCGGAGGTTTATTACGCCAGGATATATT
GTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGT
ACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCC
TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCC
TGTTTGATGGTGGTTCCA
   
Sequence Name 11-K116527-SALK_042514C-R210
GenBank Accession LN484592 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K116527-SALK_042514C-R210
TTTGTTTCACCACAATATATCCTGGCGTAATAAACCTCCGCTCATGATCAGACTTGTGAT
TAAGCCAGAGTATTTTGTGTATTGGAATTAGAACTATTATTGCAATTTCTGAGGTTTATT
CATGATTTTTCTTTGATTATACATAAACAAACTTTTGATCTTTTGTTGCTAAACTTGTTG
TTCTTGCTGGGGTATGCAGTTCGCCTGGTCAGACGTACCAAATCCAACACTGTACTCCTT
TCTGGGCTGACCGGAAGTGGAAAGACTGTGCTCTTTTACCAAGTGAGTGTTTTGGCTTGA
CGTTACCTTTTTCATATGAATATTGTTTATGACTTTCCTTCAAATGTACTTTTTTTTTGT
AATGTGTCACTATGGTGAGTCAGTGTGAGTGACTAGGTCATTGTTGGAGAGTTATAACAC
GATAATTGTTTGATTGGCAGCTCCAAGATGGATCGTCGCATCAGGGCACTGTAACATCAA
TGGACCAAAAATGAAG

Last Updated on 06.12.2018 16:11