Genotyping and primer details


Genotyping and primer details for SALK_042719c - At2g27070
Line ID SALK_042719c
Locus At2g27070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11560136 of line SALK_042719c Chr2:11560136
   
Insertion allele amplicon LBb3 - 17ty
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 17ty: 5'-ATGTCGTATGCAAACACGATCTAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11559629
   
wt amplicon (over InsPos) 27uu - 17ty
wt amplicon start Chr2:11559629
wt amplicon end Chr2:11560519
wt amplicon size 891 bp
Locus specific primer 17ty: 5'-ATGTCGTATGCAAACACGATCTAC-3'
2nd locus specific primer 27uu: 5'-ACGCTATTTCTGAGCAATGGTAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11