Genotyping and primer details


Genotyping and primer details for SALK_042723 - At4g02350
Line ID SALK_042723
Locus At4g02350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:1038674 of line SALK_042723 Chr4:1038674
   
Insertion allele amplicon LBb3 - 14rb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 14rb: 5'-AATCGGAGATAAAACAATGCAATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1038141
   
wt amplicon (over InsPos) 19rz - 14rb
wt amplicon start Chr4:1038141
wt amplicon end Chr4:1039037
wt amplicon size 897 bp
Locus specific primer 14rb: 5'-AATCGGAGATAAAACAATGCAATC-3'
2nd locus specific primer 19rz: 5'-GGTGTCAAATCAAAACCTAGCAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11