Genotyping and primer details


Genotyping and primer details for SALK_042875c - At3g66656
Line ID SALK_042875c
Locus At3g66656 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2091641 of line SALK_042875c Chr3:2091641
   
Insertion allele amplicon LBb3 - 10rr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 10rr: 5'-AATAAACCTGTACCAACCATCTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2091147
   
wt amplicon (over InsPos) 09sx - 10rr
wt amplicon start Chr3:2091147
wt amplicon end Chr3:2092003
wt amplicon size 857 bp
Locus specific primer 10rr: 5'-AATAAACCTGTACCAACCATCTGC-3'
2nd locus specific primer 09sx: 5'-TGCAAGATTTTCAAAGTGGAGTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11