Sequences from confirmation amplicon


Confirmation sequences for line SALK_042875c and hit At3g66656 are shown.
Line ID SALK_042875c
Locus At3g66656 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2091641
Primers used for PCR 10rr - LBb3, show primer details
   
Sequence Name 45-K116392-SALK_042875C-10rr
GenBank Accession LN485398 [GenBank]
Sequencing primer gene specific primer, 10rr: 5'-AATAAACCTGTACCAACCATCTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K116392-SALK_042875C-10rr
ATCGGTGTTGTTACTGTTGCCTTCTCATTTGCATAACTGCTAATTATCATTAGAGAGAAA
CATGAGAGAAGACGAAGCCTGCATATGACTAATGTTACTTTGGATCCCACAGCTTATTTT
CTTAATCTTAGCCTCAAATTCCTTGAGTTCCTCAAGCGTAAGCTCCTCAATGGGCTTATT
GAACTTATCCTGTTCAGCAGATTCAAGCCACTTGTGAAGATCTTCACCATGTTTCTTCTC
AGCTTCAGCTTCTTCAGTAATCGAGTTGAGGCGTTTACATATCTTCTTCTCCTGTCTAGC
TCTATTGCCTCTACTATAGCCTGATGTTTCACAGCTATCTCCTTCTTCTTCTTCTTCGAA
TTCATTCTTAAACCGTTCTGCAATCACATCAAAATTCGGTTTCCCGAAGGAATACGGTTT
GTTTCCTGGAGAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAA
TGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACC
GCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAA
TCCTGTTTGATGG
   
Sequence Name 20-K116391-SALK_042875C-R210
GenBank Accession LN484859 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K116391-SALK_042875C-R210
GAACAACCGTATTCCTTCGGGAAACCGAATTTTGATGTGATTGCAGAACGGTTTAAGAAT
GAATTCGAAGAAGAAGAAGAAGGAGATAGCTGTGAAACATCAGGCTATAGTAGAGGCAAT
AGAGCTAGACAGGAGAAGAAGATATGTAAACGCCTCAACTCGATTACTGAAGAAGCTGAA
GCTGAGAAGAAACATGGTGAAGATCTTCACAAGTGGCTTGAATCTGCTGAACAGGATAAG
TTCAATAAGCCCATTGAGGAGCTTACGCTTGAGGAACTCAAGGAATTTGAGGCTAAGATT
AAGAAAATAAGCTGTGGGATCCAAAGTAACATTAGTCATATGCAGGCTTCGTCTTCTCTC
ATGTTTCTCTCTAATGATAATTAGCAGTTATGCAAATGAGAAGGCAACAGTAACAACACC
GATTTGGAACTTTCATTTTGTATTAGTCGTTATAGATTTATATGGTAGAGATGTAGCAGA
TGGTTGGTACAGGTTTATT

Last Updated on 06.12.2018 16:11