Genotyping and primer details

Genotyping and primer details for SALK_043125 - At4g26660
Line ID SALK_043125
Locus At4g26660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13450636 of line SALK_043125 Chr4:13450636
Insertion allele amplicon LBb3 - 67kh
Locus specific primer 67kh: 5'-GTCGTCGATTCTCCTATGTCTTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13450227
wt amplicon (over InsPos) 13mc - 67kh
wt amplicon start Chr4:13450227
wt amplicon end Chr4:13450936
wt amplicon size 710 bp
Locus specific primer 67kh: 5'-GTCGTCGATTCTCCTATGTCTTTC-3'
2nd locus specific primer 13mc: 5'-GCTAGATCATCACTGTTCTCACCA-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11