Sequences from confirmation amplicon


Confirmation sequences for line SALK_043125 and hit At4g26660 are shown.
Line ID SALK_043125
Locus At4g26660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13450636
Primers used for PCR 67kh - LBb3, show primer details
   
Sequence Name 70-K113949-SALK_043125C-67kh
GenBank Accession LN486008 [GenBank]
Sequencing primer gene specific primer, 67kh: 5'-GTCGTCGATTCTCCTATGTCTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K113949-SALK_043125C-67kh
AAAACCTGAGATATTTGCCTACTTTCTCTATAGCTTTAATTTTGGTAATTCTGTTTCGTT
ACTTATCTTATAATCTCTTCTATATCAGGTAACAGAAGAAGCTGGTTCAAACGAAAAACA
TCTCAAGAACATGTGTATGGAGCAAGCAGCTAAAATTGAGGAGCTAACTCTCCTGGTAAA
AACCTTGTAAACTTTAAAGAATATTAGTGTTATAAAACCTTGAATCAGATTTTCAAAAAT
TAGTTTTGATAATTCTTAACTTAGACCAATATACAACATAGCTGTGGAAGAGCCTGTGAA
GTTACCAGTTGAGTTTCATTGACGATATTTGACAAGAATGGCAATCTAAACAAATTGACG
CTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTT
CACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAG
CAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 59-K113948-SALK_043125C-R210
GenBank Accession LN485719 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K113948-SALK_043125C-R210
ATTGCCATTCTTGTCAATATCGTCAATGAAACTCAACTGGTAACTTCACAGGCTCTTCCA
CAGCTATGTTGTATATTGGTCTAAGTTAAGAATTATCAAAACTAATTTTTGAAAATCTGA
TTCAAGGTTTTATAACACTAATATTCTTTAAAGTTTACAAGGTTTTTACCAGGAGAGTTA
GCTCCTCAATTTTAGCTGCTTGCTCCATACACATGTTCTTGAGATGTTTTTCGTTTGAAC
CAGCTTCTTCTGTTACCTGATATAGAAGAGATTATAAGATAAGTAACGAAACAGAATTAC
CAAAATTAAAGCTATAGAGAAAGTAGGCAAATATCTCAGGTTTTGTGGACATACTTCAGC
TTCTTGGTCAACTTTCTTTCTACATGAAAGACATAGGAAATTCGACGA

Last Updated on 06.12.2018 16:11