Genotyping and primer details


Genotyping and primer details for SALK_043673 - At1g73230
Line ID SALK_043673
Locus At1g73230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27541104 of line SALK_043673 Chr1:27541104
   
Insertion allele amplicon LBb3 - 71xo
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 71xo: 5'-GAAATCGATTAACAAGGTAAGCCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27541835
   
Insertion allele amplicon R204 - BI24
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer BI24: 5'-CTTCTTCCTCCTCCTCTAAGAAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27540491
   
wt amplicon (over InsPos) BI25 - BI24
wt amplicon start Chr1:27540491
wt amplicon end Chr1:27541349
wt amplicon size 859 bp
Locus specific primer BI24: 5'-CTTCTTCCTCCTCCTCTAAGAAGC-3'
2nd locus specific primer BI25: 5'-TTGATGAAGATGGCTAACACTGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11