Genotyping and primer details


Genotyping and primer details for SALK_043690c - At5g54680
Line ID SALK_043690c
Locus At5g54680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22217056 of line SALK_043690c Chr5:22217056
   
Insertion allele amplicon LBb3 - 22ea
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 22ea: 5'-ATGTTCTCGAATATTATCGGTTCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22216315
   
wt amplicon (over InsPos) 16fg - 22ea
wt amplicon start Chr5:22216315
wt amplicon end Chr5:22217433
wt amplicon size 1119 bp
Locus specific primer 22ea: 5'-ATGTTCTCGAATATTATCGGTTCG-3'
2nd locus specific primer 16fg: 5'-GCACTTAAGAGAGAAAGGGGAAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11