Genotyping and primer details


Genotyping and primer details for SALK_043724c - At1g54360
Line ID SALK_043724c
Locus At1g54360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20292317 of line SALK_043724c Chr1:20292317
   
Insertion allele amplicon LBb3 - 38wu
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 38wu: 5'-AATTATCCAATCTTGTTGGCTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20291706
   
wt amplicon (over InsPos) 18xf - 38wu
wt amplicon start Chr1:20291706
wt amplicon end Chr1:20292843
wt amplicon size 1138 bp
Locus specific primer 38wu: 5'-AATTATCCAATCTTGTTGGCTTTG-3'
2nd locus specific primer 18xf: 5'-TTATTTAAGAACGCGTATCTCCGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11