Genotyping and primer details


Genotyping and primer details for SALK_044034c - At5g66500
Line ID SALK_044034c
Locus At5g66500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26548006 of line SALK_044034c Chr5:26548006
   
Insertion allele amplicon LBb3 - 17gg
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 17gg: 5'-AGGAATGTTTTGGTATGATGAAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26548464
   
Insertion allele amplicon LBb3 - 19ql
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 19ql: 5'-CTCTTGAAATCTTCAGGGAAATGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26548578
   
wt amplicon (over InsPos) 05ho - 17gg
wt amplicon start Chr5:26547760
wt amplicon end Chr5:26548464
wt amplicon size 705 bp
Locus specific primer 17gg: 5'-AGGAATGTTTTGGTATGATGAAGG-3'
2nd locus specific primer 05ho: 5'-TGTGGTTTTGATCAATTCTCTCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11