Sequences from confirmation amplicon


Confirmation sequences for line SALK_044034c and hit At5g66500 are shown.
Line ID SALK_044034c
Locus At5g66500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26548006
Primers used for PCR 19ql - LBb3, show primer details
   
Sequence Name 96-K115461-SALK_044034C-19ql
GenBank Accession LN486360 [GenBank]
Sequencing primer gene specific primer, 19ql: 5'-CTCTTGAAATCTTCAGGGAAATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>96-K115461-SALK_044034C-19ql
TTTCTTGTTGTTATATCGGCTTGTGCACACGCAGGACTAGTTAAAGAAGGTAAGGAATGT
TTTGGTATGATGAAGGAGAAGTATCGGTTGGTTCCTGGAACAGAGCATTACGTATGCTTC
ATCGATATCTTAAGCAAGGCTGGTGAGACAGAAGAGATATGGAGATTAGTCGAGAGAATG
ATGGAGAACGATAATCAAAGCATTCCTTGTGCTATATGGGTAGCGGTACTCAGTGCTTGT
AGTCTTAATATGGATCTTACGCGAGGCGAATATGTAGCAAGGAGGCTTATGGAAGAGACG
GGTCCAGAGAACGCGAGCATTTATGTGTTGGTTTCGAATTTCTATGCAGCGATGGGGAAG
TGGGATGTCGTTGAAGAATTGAGAGGAAAACTGAAGAATAAAGGTTTGGTTAAAACAGCA
GGACACAGCTTATTCATATGAATTGCTTTGTTTTTTCAGCATTACACTACACTTGAGCTA
CTGTTTTTGTGTCAAGAAACAGAGCAAGTCCACTTAATAACACATTGACGCTTAGACAAC
TTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAG
ACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCC
ACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAA
   
Sequence Name 94-K115460-SALK_044034C-R210
GenBank Accession LN486357 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K115460-SALK_044034C-R210
TGGACTTGCTCTGTTTCTTGACACAAAAACAGTAGCTCAAGTGTAGTGTAATGCTGAAAA
AACAAAGCAATTCATATGAATAAGCTGTGTCCTGCTGTTTAACCAAACCTTTATTCTTCA
GTTTTCCTCTCAATTCTTCAACGACATCCCACTTCCCCATCGCTGCATAGAAATTCGAAA
CCAACACATAAATGCTCGCGTTCTCTGGACCCGTCTCTTCCATAAGCCTCCTTGCTACAT
ATTCGCCTCGCGTAAGATCCATATTAAGACTACAAGCACTGAGTACCGCTACCCATATAG
CACAAGGAATGCTTTGATTATCGTTCTCCATCATTCTCTCGACTAATCTCCATATCTCTT
CTGTCTCACCAGCCTTGCTTAAGATATCGATGAAGCATACGTAATGCTCTGTTCCAGGAA
CCAACCGATACTTCTCCTTCATCATACCAAAACATTCCTTACCTTCTTTAACTAGTCCTG
CGTGTGCACAAGCCGATATAACAACAAGAAATGTCACTGAATTCGGTAAAACTCCGCTTC
CTTCTTCACACATTTCCCTGA
Primers used for PCR 17gg - LBb3, show primer details
   
Sequence Name 37-K115497-SALK_044034C-17gg
GenBank Accession LN485251 [GenBank]
Sequencing primer gene specific primer, 17gg: 5'-AGGAATGTTTTGGTATGATGAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K115497-SALK_044034C-17gg
GTATGCTTCATCGATATCTTAGCAAGGCTGGTGAGACAGAAGAGATATGGAGATTAGTCG
AGAGAATGATGGAGAACGATAATCAAAGCATTCCTTGTGCTATATGGGTAGCGGTACTCA
GTGCTTGTAGTCTTAATATGGATCTTACGCGAGGCGAATATGTAGCAAGGAGGCTTATGG
AAGAGACGGGTCCAGAGAACGCGAGCATTTATGTGTTGGTTTCGAATTTCTATGCAGCGA
TGGGGAAGTGGGATGTCGTTGAAGAATTGAGAGGAAAACTGAAGAATAAAGGTTTGGTTA
AAACAGCAGGACACAGCTTATTCATATGAATTGCTTTGTTTTTTCAGCATTACACTACAC
TTGAGCTACTGTTTTTGTGTCAAGAAACAGAGCAAGTCCACTTAATAACACATTGACGCT
TAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCA
CCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCA
AGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 26-K115496-SALK_044034C-R210
GenBank Accession LN485038 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K115496-SALK_044034C-R210
GGACTTGCTCTGTTTCTTGACACAAAAACAGTAGCTCAAGTGTAGTGTAATGCTGAAAAA
ACAAAGCAATTCATATGAATAAGCTGTGTCCTGCTGTTTTAACCAAACCTTTATTCTTCA
GTTTTCCTCTCAATTCTTCAACGACATCCCACTTCCCCATCGCTGCATAGAAATTCGAAA
CCAACACATAAATGCTCGCGTTCTCTGGACCCGTCTCTTCCATAAGCCTCCTTGCTACAT
ATTCGCCTCGCGTAAGATCCATATTAAGACTACAAGCACTGAGTACCGCTACCCATATAG
CACAAGGAATGCTTTGATTATCGTTCTCCATCATTCTCTCGACTAATCTCCATATCTCTT
CTGTCTCACCAGCCTTGCTTAAGATATCGATGAAGCATACGTAATGCTCTGTTCCAGGAA
CCAACCGATACTTCTCCTTCATCATACCAAAACATTC

Last Updated on 06.12.2018 16:11