Genotyping and primer details


Genotyping and primer details for SALK_044671c - At4g15640
Line ID SALK_044671c
Locus At4g15640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:8919426 of line SALK_044671c Chr4:8919426
   
Insertion allele amplicon LBb3 - 43kq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 43kq: 5'-TGTTATCAGGTGATACAGAGTCGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8919892
   
wt amplicon (over InsPos) 17ls - 43kq
wt amplicon start Chr4:8919101
wt amplicon end Chr4:8919892
wt amplicon size 792 bp
Locus specific primer 43kq: 5'-TGTTATCAGGTGATACAGAGTCGC-3'
2nd locus specific primer 17ls: 5'-GAGGCTTACAAAACAGGACAACTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11