Genotyping and primer details


Genotyping and primer details for SALK_044963c - At1g74910
Line ID SALK_044963c
Locus At1g74910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28137136 of line SALK_044963c Chr1:28137136
   
Insertion allele amplicon LBb3 - 51dl
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 51dl: 5'-GCTTCTTCTCACATCTGAGTTCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28137652
   
wt amplicon (over InsPos) 13ec - 51dl
wt amplicon start Chr1:28136768
wt amplicon end Chr1:28137652
wt amplicon size 885 bp
Locus specific primer 51dl: 5'-GCTTCTTCTCACATCTGAGTTCCT-3'
2nd locus specific primer 13ec: 5'-TATATAGCCGTTTCTTCCCAGCTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11