Genotyping and primer details


Genotyping and primer details for SALK_045607c - At1g48605
Line ID SALK_045607c
Locus At1g48605 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:17969744 of line SALK_045607c Chr1:17969744
   
Insertion allele amplicon LBb3 - 19ty
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 19ty: 5'-TTCTGATTTGACAAAAGATGATCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:17970208
   
wt amplicon (over InsPos) 27vb - 19ty
wt amplicon start Chr1:17969202
wt amplicon end Chr1:17970208
wt amplicon size 1007 bp
Locus specific primer 19ty: 5'-TTCTGATTTGACAAAAGATGATCG-3'
2nd locus specific primer 27vb: 5'-GTTTCGATTCTTACTCGAGTCGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11