Genotyping and primer details


Genotyping and primer details for SALK_045660c - At1g25240
Line ID SALK_045660c
Locus At1g25240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8845835 of line SALK_045660c Chr1:8845835
   
Insertion allele amplicon LBb3 - 34cv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 34cv: 5'-AACTAAACGAAAAACCGGTTACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8845082
   
wt amplicon (over InsPos) 42lt - 34cv
wt amplicon start Chr1:8845082
wt amplicon end Chr1:8846205
wt amplicon size 1124 bp
Locus specific primer 34cv: 5'-AACTAAACGAAAAACCGGTTACAA-3'
2nd locus specific primer 42lt: 5'-CCATTTATCGGTTATGATTGTCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11