Genotyping and primer details


Genotyping and primer details for SALK_045920c - At3g17910
Line ID SALK_045920c
Locus At3g17910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6133520 of line SALK_045920c Chr3:6133520
   
Insertion allele amplicon LBb3 - 38fx
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 38fx: 5'-CATTGCGTCAGCATAGATCAGTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6134182
   
wt amplicon (over InsPos) 06hb - 38fx
wt amplicon start Chr3:6133311
wt amplicon end Chr3:6134182
wt amplicon size 872 bp
Locus specific primer 38fx: 5'-CATTGCGTCAGCATAGATCAGTAT-3'
2nd locus specific primer 06hb: 5'-ATAGAGACAGAAGACGCAACCATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11