Sequences from confirmation amplicon


Confirmation sequences for line SALK_045920c and hit At3g17910 are shown.
Line ID SALK_045920c
Locus At3g17910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6133520
Primers used for PCR 38fx - LBb3, show primer details
   
Sequence Name 61-K111812-SALK_045920C-38fx
GenBank Accession LN485765 [GenBank]
Sequencing primer gene specific primer, 38fx: 5'-CATTGCGTCAGCATAGATCAGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K111812-SALK_045920C-38fx
CTCCGGATCTAGTTCTCTAACTCCTTTACGGATCACAAAAACAACTAGAATATGTGTGAA
GCACATTTTGAATATAAATTTCAATTAAAAGTCTAAGACAGGCTATAACCAAGCTTGAGA
CGATGAAAGGACTTCCATTGATTTCGAAGTACTGACCTTTTCCTCTCTCCTGACGATCTG
CCATGACCCGAGACCAAAGGTAATCGCTCCAGGCAAGAACAACAACAATTGTGACCACTT
AGACCCTCGTTTGTTCTCTGAAACAAAAAGGGCTTAGCTTTTCAGAAATTGATGCTTACT
ATCCTCAGATTCCAAGCTCAAGCATTGGAAGACATAGCACGAGCAACATACCTTGAGGTG
GAGCAGAAGAACTCGATTGGCTTCCAAGAGCGGCGGAGCTAGAGGAGGAAGAGTCAGCGA
CGGCAGAGAAGTGTCTTGACCAGAATTGTTTCGGTAAAGAAGGAGAGGCAGAGATACTTG
TTGTAGTAGAGCACCAGTATCTCTTAGTGTTCGATCTCGTTAGGATTTTGGAAAGCGAGG
TCGCCATCTTCGTCTCTCCCCAAGATTTTGATCCCGGAGGTAAAGGGTTTTTGCCTCCAC
GACGACTTTGGAATCACCAGCCAGCTATAACACAAATCAATTGACGCTTAGACAACTTAA
TAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGG
GCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGC
TGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 51-K111811-SALK_045920C-R210
GenBank Accession LN485534 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K111811-SALK_045920C-R210
GCTGGCTGGTGATTCCAAGTCGTCGTGGAGGCAAAAACCCTTTACCTCCGGGATCAAAAT
CTTGGGGAGAGACGAAGATGGCGACCTCGCTTTCCAAAATCCTAACGAGATCGAACACTA
AGAGATACTGGTGCTCTACTACAACAAGTATCTCTGCCTCTCCTTCTTTACCGAAACAAT
TCTGGTCAAGACACTTCTCTGCCGTCGCTGACTCTTCCTCCTCTAGCTCCGCCGCTCTTG
GAAGCCAATCGAGTTCTTCTGCTCCACCTCAAGGTATGTTGCTCGTGCTATGTCTTCCAA
TGCTTGAGCTTGGAATCTGAGGATAGTAAGCATCAATTTCTGAAAAGCTAAGCCCTTTTT
GTTTCAGAGAACAAACGAGGGTCTAAGTGGTCACAATTGTTGTTGTTCTTGCCTGGAGCG
ATTACCTTTGGTCTCGGGTCATGGCAGATCGTCAGGAGAGAGGAAAAGGTCAGTACTTCG
AAATCAATGGAAGTCCTTTCATCGTCTCAAGCTTGGTTATAGCCTGTCTTAGACTTTTAA
TTGAAATTTATATTCAAAATGTGCTTCACACATATTCTAGTTGTTTTTGTGATCCGTAAA
GGAGTTAGAGTAACTAGATCCTGGAGTCGTAATCTGTGTGATCTGGATAGAGCACATACT
GATCTAT

Last Updated on 06.12.2018 16:11