Genotyping and primer details


Genotyping and primer details for SALK_046054 - At1g01230
Line ID SALK_046054
Locus At1g01230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:98083 of line SALK_046054 Chr1:98083
   
Insertion allele amplicon LBb3 - 11rr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 11rr: 5'-CTCATGTTGGTTTTATGTTCCCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:97310
   
wt amplicon (over InsPos) 25tf - 11rr
wt amplicon start Chr1:97310
wt amplicon end Chr1:98525
wt amplicon size 1216 bp
Locus specific primer 11rr: 5'-CTCATGTTGGTTTTATGTTCCCTT-3'
2nd locus specific primer 25tf: 5'-ACCATTGTAGATTCCTTGGTCATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11