Genotyping and primer details


Genotyping and primer details for SALK_046315c - At4g14150
Line ID SALK_046315c
Locus At4g14150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:8162132 of line SALK_046315c Chr4:8162132
   
Insertion allele amplicon LBb3 - 60fr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 60fr: 5'-GGAAACTGGACTCGTGATCTAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8161089
   
wt amplicon (over InsPos) 02hs - 60fr
wt amplicon start Chr4:8161089
wt amplicon end Chr4:8162210
wt amplicon size 1122 bp
Locus specific primer 60fr: 5'-GGAAACTGGACTCGTGATCTAAAG-3'
2nd locus specific primer 02hs: 5'-GAAGTCTTCAGACCTCAAAAATGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11