Genotyping and primer details


Genotyping and primer details for SALK_046603c - At3g56600
Line ID SALK_046603c
Locus At3g56600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20969411 of line SALK_046603c Chr3:20969411
   
Insertion allele amplicon LBb3 - 39vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 39vi: 5'-TTGTTTTGAATTTGCAGAAACTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20968793
   
wt amplicon (over InsPos) 52vw - 39vi
wt amplicon start Chr3:20968793
wt amplicon end Chr3:20970227
wt amplicon size 1435 bp
Locus specific primer 39vi: 5'-TTGTTTTGAATTTGCAGAAACTTG-3'
2nd locus specific primer 52vw: 5'-CCAACACACTAGAACTCTCTTCCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11