Genotyping and primer details


Genotyping and primer details for SALK_046675c - At2g47460
Line ID SALK_046675c
Locus At2g47460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:19478828 of line SALK_046675c Chr2:19478828
   
Insertion allele amplicon LBb3 - 08ei
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 08ei: 5'-ACCTTACGTACAATCCAAACATCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19479403
   
wt amplicon (over InsPos) 26gc - 08ei
wt amplicon start Chr2:19478417
wt amplicon end Chr2:19479403
wt amplicon size 987 bp
Locus specific primer 08ei: 5'-ACCTTACGTACAATCCAAACATCC-3'
2nd locus specific primer 26gc: 5'-AGGGAGAACAGACAACGAAATAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11