Genotyping and primer details


Genotyping and primer details for SALK_046841c - At3g18660
Line ID SALK_046841c
Locus At3g18660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6421144 of line SALK_046841c Chr3:6421144
   
Insertion allele amplicon LBb3 - 25dh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 25dh: 5'-TAAGCAGAGAAGCAATATGCAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6421801
   
wt amplicon (over InsPos) 02dz - 25dh
wt amplicon start Chr3:6420701
wt amplicon end Chr3:6421801
wt amplicon size 1101 bp
Locus specific primer 25dh: 5'-TAAGCAGAGAAGCAATATGCAAGA-3'
2nd locus specific primer 02dz: 5'-AGCTTGGCCTATCTGTAAAATTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11