Genotyping and primer details


Genotyping and primer details for SALK_047206c - At1g68380
Line ID SALK_047206c
Locus At1g68380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:25636594 of line SALK_047206c Chr1:25636594
   
Insertion allele amplicon LBb3 - 54cs
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 54cs: 5'-AGGCTTTTGTGTTTTCTCAATGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25635717
   
wt amplicon (over InsPos) 41lt - 54cs
wt amplicon start Chr1:25635717
wt amplicon end Chr1:25637098
wt amplicon size 1382 bp
Locus specific primer 54cs: 5'-AGGCTTTTGTGTTTTCTCAATGTT-3'
2nd locus specific primer 41lt: 5'-TGGAAGATAATGAACTTCTGTGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11