Genotyping and primer details


Genotyping and primer details for SALK_047290c - At2g21040
Line ID SALK_047290c
Locus At2g21040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9026580 of line SALK_047290c Chr2:9026580
   
Insertion allele amplicon R212 - 72xo
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer 72xo: 5'-CAAGTTTTCCAAGGAATAGTGGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9027547
   
Insertion allele amplicon R204 - BI66
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer BI66: 5'-TATGCAAGTCAACTTCACCATCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9025558
   
wt amplicon (over InsPos) BI67 - BI66
wt amplicon start Chr2:9025558
wt amplicon end Chr2:9026764
wt amplicon size 1207 bp
Locus specific primer BI66: 5'-TATGCAAGTCAACTTCACCATCTC-3'
2nd locus specific primer BI67: 5'-AAGTAGAGCCATATCTCCAAACGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11