Genotyping and primer details


Genotyping and primer details for SALK_047557c - At1g29820
Line ID SALK_047557c
Locus At1g29820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:10438426 of line SALK_047557c Chr1:10438426
   
Insertion allele amplicon LBb3 - 35cv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 35cv: 5'-TGAGATTTTGGTTTCTCGAAAGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:10437700
   
wt amplicon (over InsPos) 04dz - 35cv
wt amplicon start Chr1:10437700
wt amplicon end Chr1:10438627
wt amplicon size 928 bp
Locus specific primer 35cv: 5'-TGAGATTTTGGTTTCTCGAAAGTT-3'
2nd locus specific primer 04dz: 5'-ACTCAGCGTTTACTTGAACCATTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11