Genotyping and primer details


Genotyping and primer details for SALK_048014c - At5g18130
Line ID SALK_048014c
Locus At5g18130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5996184 of line SALK_048014c Chr5:5996184
   
Insertion allele amplicon LBb3 - 15vo
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 15vo: 5'-CAAATGTGAGATTACACACCCATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5996505
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11