Sequences from confirmation amplicon


Confirmation sequences for line SALK_048014c and hit At5g18130 are shown.
Line ID SALK_048014c
Locus At5g18130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5996184
Primers used for PCR 15vo - LBb3, show primer details
   
Sequence Name 82-K118049-SALK_048014C-15vo
GenBank Accession LN486248 [GenBank]
Sequencing primer gene specific primer, 15vo: 5'-CAAATGTGAGATTACACACCCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K118049-SALK_048014C-15vo
TTCGTATCATAAAAATTAATTTTCCTTAACTAAGTGACTTCTAACAAGAATCATATTTTA
AATTTTTTACCTTATTATCATCAGAGTAAATCTGGAACCTGAGGATCTGGTTCTTGTTCC
GACCACCTTGGATGCTACTGTTGTGTCCAAGGAAGAAGACACATATGGTGGCTGCAGCAG
CTGCAACACCAAATGAACATATAGCTCCAATTCCATTAAGACCCTTCTTCCACAGATTCG
TTCTTTCACCTTCATCCCAATCAACCTCTTTCTTGCTACTCAAGTCACTGCGGACAAATT
GACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTC
TTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTT
GCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
C
   
Sequence Name 76-K118048-SALK_048014C-R210
GenBank Accession LN486161 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K118048-SALK_048014C-R210
AAAGAACGAATCTGTGGAAGAAGGGTCTTAATGGAATTGGAGCTATATGTTCATTTGGTG
TTGCAGCTGCTGCAGCCACCATATGTGTCTTCTTCCTTGGACACAACAGTAGCATCCAAG
GTGGTCGGAACAAGAACCAGATCCTCAGGTTCCAGATTTACTCTGATGATAATAAGGTAA
AAAATTTAAAATATGATTCTTGTTAGAAGTCACTTAGTTAAGGAAAATTAATTTTTATGA
TACGAAATACACTAATTTTGATAACTGATATATCTGATTAGTTCTACTAGTTTAGTTAGA
TGGGTGTGTATCTTCCACATTTG

Last Updated on 06.12.2018 16:11