Genotyping and primer details


Genotyping and primer details for SALK_048033 - At4g13460
Line ID SALK_048033
Locus At4g13460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:7826256 of line SALK_048033 Chr4:7826256
   
Insertion allele amplicon LBb3 - 09ef
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 09ef: 5'-TGGATTTAGCTTGAAAGGTGTTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:7826699
   
wt amplicon (over InsPos) 12gc - 09ef
wt amplicon start Chr4:7826030
wt amplicon end Chr4:7826699
wt amplicon size 670 bp
Locus specific primer 09ef: 5'-TGGATTTAGCTTGAAAGGTGTTCT-3'
2nd locus specific primer 12gc: 5'-ATCTCTCATCATTGAACCAGCTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11