Genotyping and primer details


Genotyping and primer details for SALK_048245c - At5g53750
Line ID SALK_048245c
Locus At5g53750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:21817389 of line SALK_048245c Chr5:21817389
   
Insertion allele amplicon LBb3 - 23ea
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 23ea: 5'-AATCAGATTCTGTGCTCCTTTGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13733462
   
wt amplicon (over InsPos) 04fi - 23ea
wt amplicon start Chr4:13733462
wt amplicon end Chr5:21816883
wt amplicon size 1004 bp
Locus specific primer 23ea: 5'-AATCAGATTCTGTGCTCCTTTGAT-3'
2nd locus specific primer 04fi: 5'-TCATAAGAGAAGGACATGATCAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11