Genotyping and primer details


Genotyping and primer details for SALK_049248c - At1g75990
Line ID SALK_049248c
Locus At1g75990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28524769 of line SALK_049248c Chr1:28524769
   
Insertion allele amplicon LBb3 - 48cy
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 48cy: 5'-GTGAATCTGAGAACCTGTGATGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28524048
   
wt amplicon (over InsPos) 40lt - 48cy
wt amplicon start Chr1:28524048
wt amplicon end Chr1:28525065
wt amplicon size 1018 bp
Locus specific primer 48cy: 5'-GTGAATCTGAGAACCTGTGATGAA-3'
2nd locus specific primer 40lt: 5'-ACAGGACTCACAACCTCATAGTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11