Genotyping and primer details


Genotyping and primer details for SALK_049398 - At3g10730
Line ID SALK_049398
Locus At3g10730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3358484 of line SALK_049398 Chr3:3358484
   
Insertion allele amplicon LBb3 - 42vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 42vi: 5'-GACAATATGCATTGAGGAGGATTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3359221
   
wt amplicon (over InsPos) 49vw - 42vi
wt amplicon start Chr3:3357892
wt amplicon end Chr3:3359221
wt amplicon size 1330 bp
Locus specific primer 42vi: 5'-GACAATATGCATTGAGGAGGATTT-3'
2nd locus specific primer 49vw: 5'-TAGCTCAATTTGTTATGCCAGTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11