Genotyping and primer details


Genotyping and primer details for SALK_049590c - At5g22400
Line ID SALK_049590c
Locus At5g22400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:7425315 of line SALK_049590c Chr5:7425315
   
Insertion allele amplicon LBb3 - 73hw
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 73hw: 5'-AGGTTCTACAACTCGAAACAAGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7424720
   
wt amplicon (over InsPos) 01kj - 73hw
wt amplicon start Chr5:7424720
wt amplicon end Chr5:7425418
wt amplicon size 699 bp
Locus specific primer 73hw: 5'-AGGTTCTACAACTCGAAACAAGGA-3'
2nd locus specific primer 01kj: 5'-TTCCTTCAATTCAAAACCTTTTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11