Genotyping and primer details

Genotyping and primer details for SALK_049953 - At1g16680
Line ID SALK_049953
Locus At1g16680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5703759 of line SALK_049953 Chr1:5703759
Insertion allele amplicon LBb3 - 12vt
Locus specific primer 12vt: 5'-CTAAAACAAGGGTAACACAGCACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5704250
wt amplicon (over InsPos) 70wi - 12vt
wt amplicon start Chr1:5702944
wt amplicon end Chr1:5704250
wt amplicon size 1307 bp
Locus specific primer 12vt: 5'-CTAAAACAAGGGTAACACAGCACA-3'
2nd locus specific primer 70wi: 5'-ATTGGTTAAGCAAGGTTGGATATG-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11