Sequences from confirmation amplicon


Confirmation sequences for line SALK_049953 and hit At1g16680 are shown.
Line ID SALK_049953
Locus At1g16680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5703759
Primers used for PCR 12vt - LBb3, show primer details
   
Sequence Name 76-K118240-SALK_049953C-12vt
GenBank Accession LN486162 [GenBank]
Sequencing primer gene specific primer, 12vt: 5'-CTAAAACAAGGGTAACACAGCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K118240-SALK_049953C-12vt
AGTTTCCAAGCCAAGAAAACAGACCTTCTTTCTATAGTCTTTCTTAAGCAATGCGGCATC
AATCTTTTTAAACAGAGGGAGCCCCAATGCTTCATAGTGATTCAAACTATCAAGAATTCG
TTTCATCTCATCCGCTGAATCTGTTTCTATTTTGACTACCTTAACAGTAGAAATCTCCTT
CATGTTACTGACAACAGTTGACGATGATGCTGGCTTAGTGGACGACTTATCTTCATGAAC
CTTTTTCTCTGCTTCTTCAGCAGGAACAGAAGAATACTCAAACTCTCCTGGAAACTCTTC
GTCTATTACAGTTTCAGGTTTCGTTGGCTCCTCCACTTGTGTTTTTTCACTAACAGTATC
ACACCATTGAAGAAGACAAATAAATATATCGTTGGACAAGAATGCAAGATTCATCGAGAG
AATAACTCCAGGCCCAACGTTGACGCTTAAACAAATTGACGCTTAGACAACTTAATAACA
CATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAAC
AGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTT
TGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 53-K118239-SALK_049953C-R210
GenBank Accession LN485591 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K118239-SALK_049953C-R210
TTTGTTTAGCGTCACGTTGGGCCTGGAGTTATTCTCTCGATGAATCTTGCATTCTTGTCC
AACGATATATTTATTTGTCTTCTTCAATGGTGTGATACTGTTAGTGAAAAAACACAAGTG
GAGGAGCCAACGAAACCTGAAACTGTAATAGACGAAGAGTTTCCAGGAGAGTTTGAGTAT
TCTTCTGTTCCTGCTGAAGAAGCAGAGAAAAAGGTTCATGAAGATAAGTCGTCCACTAAG
CCAGCATCATCGTCAACTGTTGTCAGTAACATGAAGGAGATTTCTACTGTTAAGGTAGTC
AAAATAGAAACAGATTCAGCGGATGAGATGAAACGAATTCTTGATAGTTTGAATCACTAT
GAAGCATTGGGGCTCCCTCTGTTTAAAAAGATTGATGCCGCATTGCTTAAGAAAGACTAT
AGAAAGAAGGTCTGTTTTCTTGGCTTGGAAACTATGTCTCTGGTGAATTAAGTTGGTTCT
AATTTTATGTGCTGTGTTACCCTTGTTTTA

Last Updated on 06.12.2018 16:11