Genotyping and primer details


Genotyping and primer details for SALK_050009c - At4g03153
Line ID SALK_050009c
Locus At4g03153 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:1395745 of line SALK_050009c Chr4:1395745
   
Insertion allele amplicon R204 - BN61
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer BN61: 5'-TGTTTCCAACATTCCATATTCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1394721
   
wt amplicon (over InsPos) 39lt - BN61
wt amplicon start Chr4:1394721
wt amplicon end Chr4:1395990
wt amplicon size 1270 bp
Locus specific primer BN61: 5'-TGTTTCCAACATTCCATATTCAAA-3'
2nd locus specific primer 39lt: 5'-AAAGATTTACCACATCATCAGCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11