Genotyping and primer details


Genotyping and primer details for SALK_050669c - At3g62700
Line ID SALK_050669c
Locus At3g62700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:23190761 of line SALK_050669c Chr3:23190761
   
Insertion allele amplicon R212 - 25au
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer 25au: 5'-TCCAAATATCGAGCAGAGAGTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:23190189
   
Insertion allele amplicon R204 - 54aq
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer 54aq: 5'-TCTGATGAAGAAATCTGGAAGGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:23191102
   
wt amplicon (over InsPos) 54aq - 25au
wt amplicon start Chr3:23190189
wt amplicon end Chr3:23191102
wt amplicon size 914 bp
Locus specific primer 25au: 5'-TCCAAATATCGAGCAGAGAGTTTT-3'
2nd locus specific primer 54aq: 5'-TCTGATGAAGAAATCTGGAAGGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11