Genotyping and primer details


Genotyping and primer details for SALK_050811 - At2g33340
Line ID SALK_050811
Locus At2g33340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14126888 of line SALK_050811 Chr2:14126888
   
Insertion allele amplicon LBb3 - 79by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 79by: 5'-CCTGGTCACAATAAAGAAAGGGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14125993
   
wt amplicon (over InsPos) 38lt - 79by
wt amplicon start Chr2:14125993
wt amplicon end Chr2:14127263
wt amplicon size 1271 bp
Locus specific primer 79by: 5'-CCTGGTCACAATAAAGAAAGGGTA-3'
2nd locus specific primer 38lt: 5'-AATGATTTTCTTTTTGTCCTGGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11