Genotyping and primer details


Genotyping and primer details for SALK_051124c - At3g14990
Line ID SALK_051124c
Locus At3g14990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5047351 of line SALK_051124c Chr3:5047351
   
Insertion allele amplicon LBb3 - 25ea
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 25ea: 5'-TCCACTTATTGGCTGTCCTTAGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5047920
   
wt amplicon (over InsPos) 11fg - 25ea
wt amplicon start Chr3:5046980
wt amplicon end Chr3:5047920
wt amplicon size 941 bp
Locus specific primer 25ea: 5'-TCCACTTATTGGCTGTCCTTAGTT-3'
2nd locus specific primer 11fg: 5'-TAGGAGTACTTTTTCGACCGGTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11