Genotyping and primer details


Genotyping and primer details for SALK_051729 - At4g24970
Line ID SALK_051729
Locus At4g24970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:12833173 of line SALK_051729 Chr4:12833173
   
Insertion allele amplicon LBb3 - 56us
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 56us: 5'-ATCTTTGGTTAACATGGGATTCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12832746
   
wt amplicon (over InsPos) 66vl - 56us
wt amplicon start Chr4:12832746
wt amplicon end Chr4:12833700
wt amplicon size 955 bp
Locus specific primer 56us: 5'-ATCTTTGGTTAACATGGGATTCAG-3'
2nd locus specific primer 66vl: 5'-CACATCAAAGTACGAAACCTTCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11