Genotyping and primer details


Genotyping and primer details for SALK_051923c - At4g14600
Line ID SALK_051923c
Locus At4g14600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:8376624 of line SALK_051923c Chr4:8376624
   
Insertion allele amplicon LBb3 - 30jr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 30jr: 5'-AATGGACTAACCAAAATCATGAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8377228
   
wt amplicon (over InsPos) 07ll - 30jr
wt amplicon start Chr4:8376197
wt amplicon end Chr4:8377228
wt amplicon size 1032 bp
Locus specific primer 30jr: 5'-AATGGACTAACCAAAATCATGAGG-3'
2nd locus specific primer 07ll: 5'-ACCAGAGGTTCACGACTAAGTGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11