Sequences from confirmation amplicon


Confirmation sequences for line SALK_051923c and hit At4g14600 are shown.
Line ID SALK_051923c
Locus At4g14600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8376624
Primers used for PCR 30jr - LBb3, show primer details
   
Sequence Name 59-K113814-SALK_051923C-R210
GenBank Accession LN485716 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K113814-SALK_051923C-R210
CATTTGTTTACTCGCAGATCTAGGTTAACTTTATTATCAGTTTCTCGTTTTGGAATTGCT
TTTAAATTTCTTTCTCTGATCTTTTGGATAGAGAAAACTAGAGATTTATGGGTCATAGAT
TTTGATAATTTCTGCGGAACTATAGCTCTATTGATTTGTATTTGTGGCTGCAGGGAAGGG
CTTAGTACTAGAAATGCTGCAGGTTCAGAAGAAATCCAGCTGAGGATTGATCCGATGCAC
TCTGACCTGGATGATGAGATCACAGGTCTCCATGGCCAAGTCAGGCAGTTGAAAAATGTA
AGAAACCCTCCTAGTTACCAATTCTATCTCTCCTTTGTCTGTGTTTTCATTACAGCCTTT
TGGTTTTGAAAGTGGCTCTGTATATGTGTTTTTGTTCCAGAGTACTATGATAGTATGTAT
GTTCACTCATCAGTGAGTATCTGATTAATTAGTTGACTTACCAATTGAATTCTACATATT
TGATGTCTGTTATAAACCTTCCTTGCTTGTTGTTTTATATACCATACTTCTTAAATGAAT
CAGTTGACATTCTTTACGATTCCTTGTGTTTTACCTTTTTGAACATAACTTATGCCTCAT
GATTTTGTTAAGTCCA
   
Sequence Name 72-K113815-SALK_051923C-30jr
GenBank Accession LN486058 [GenBank]
Sequencing primer gene specific primer, 30jr: 5'-AATGGACTAACCAAAATCATGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K113815-SALK_051923C-30jr
AATCGTAAGAATGTCAACTGATTCATTTAAGAAGTATGGTATATAAAACAACAAGCAAGG
AAGGTTTATAACAGACATCAAATATGTAGAATTCAATTGGTAAGTCAACTAATTAATCAG
ATACTCACTGATGAGTGAACATACATACTATCATAGTACTCTGGAACAAAAACACATATA
CAGAGCCACTTTCAAAACCAAAAGGCTGTAATGAAAACACAGACAAAGGAGAGATAGAAT
TGGTAACTAGGAGGGTTTCTTACATTTTTCAACTGCCTGACTTGGCCATGGAGACCTGTG
ATCTCATCATCCAGGTCAGAGTGCATCGGATCAATCCTCAGCTGGATTTCTTCTGAACCT
GCAGCATTTCTAGTACTAAGCCCTTCCCTGCAGCCACAAATACAAATCAATAGAGCTATA
GTTCCGCAGAAATTATCAAAATCTATGACCCATAAATCTCTAGTTTTCTCTATCCNAAAG
ATCAGAGAAAGAAATTTAAAAGCAATTCCAAAACGAGAAACTGATAATAAAGTTATACCT
AGATCTGCTGATGTAAACAAATTGACGCTNAGACAACTTAATAACACATTGCGGACGTTT
TTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTT
CACCGCCNGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTNTGCCCCAGCAGGCG
AANATCCTGTTTGATGGTGGTTC

Last Updated on 06.12.2018 16:11