Genotyping and primer details


Genotyping and primer details for SALK_052372 - At5g43010
Line ID SALK_052372
Locus At5g43010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:17250336 of line SALK_052372 Chr5:17250336
   
Insertion allele amplicon LBb3 - 26ea
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 26ea: 5'-GGCTTTGAATGACTTGAATCTAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:17250842
   
wt amplicon (over InsPos) 02fi - 26ea
wt amplicon start Chr5:17249897
wt amplicon end Chr5:17250842
wt amplicon size 946 bp
Locus specific primer 26ea: 5'-GGCTTTGAATGACTTGAATCTAGG-3'
2nd locus specific primer 02fi: 5'-ATGGAAACTACATAAGCACAGCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11