Sequences from confirmation amplicon


Confirmation sequences for line SALK_052372 and hit At5g43010 are shown.
Line ID SALK_052372
Locus At5g43010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17250336
Primers used for PCR 26ea - LBb3, show primer details
   
Sequence Name 30-K110755-SALK_052372C-26ea
GenBank Accession LN485126 [GenBank]
Sequencing primer gene specific primer, 26ea: 5'-GGCTTTGAATGACTTGAATCTAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K110755-SALK_052372C-26ea
TTTGCTGTTTTTGTGGAATCGATTCTAGGGTTTTCTCTCAATTGCTTAAAGTGTTCGCTT
TTTCTTAGAGAAGAAGAGTGGTCTCTCGAATTAGTGAAATCGATTGGTGAAGTTTTGATT
TTTCACTGTGATGGAGTTTCATATGAGCATATGACTCGACTTGTGATTAGAACACTGAGT
TTTGTTGTGTTACTGTAGCTAGGGAGAACTTAAGAGGAGCTAAAAAGGAATTCAACAAAA
CCGAGGATGATCTCAAGTCTCTTCAAAGTGTTGGGCAGATTATTGGAGAAGTGCTTCGAC
CCCTGGATAATGAAAGATGTAATCATTTTTCCTCGACCTAATACCCTTCTTTTTTATCTT
TTGAGATTTTTGCTCATTGGACCCTTGGTTAAATTTTAAATGTTTTGTGTTTTAGTGATT
GTGAAAGCGAGCAGTGGTCCACGTTATGTGGTGTAAACAAATTGACGCTTAGACAACTTA
ATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACG
GGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACG
CTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAA
   
Sequence Name 22-K110754-SALK_052372C-R210
GenBank Accession LN484911 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K110754-SALK_052372C-R210
GTCATTTGTTTACCCACATAACGTGGACCACTGCTCGCTTTCACAATCACTAAAACACAA
AACATTTAAAATTTAACCAAGGGTCCAATGAGCAAAAATCTCAAAAGATAAAAAAGAAGG
GTATTAGGTCGAGGAAAAATGATTACATCTTTCATTATCCAGGGGTCGAAGCACTTCTCC
AATAATCTGCCCAACACTTTGAAGAGACTTGAGATCATCCTCGGTTTTGTTGAATTCCTT
TTTAGCTCCTCTTAAGTTCTCCCTAGCTACAGTAACACAACAAAACTCAGTGTTCTAATC
ACAAGTCGAGTCATATGCTCATATGAAACTCCATCACAGTGAAAAATCAAAACTTCACCA
ATCGATTTCACTAATTCGAGAGACCACTCTTCTTCTCTAAGAAAAAGCGAACACTTTAAG
CAATTGAGAGAAAACCCTAGAATCGATTCACACAAAAACAGCTAAATTTACGAAATCCAC
ATAGAAAAAAAGGAGCCCTAGATTCAAGTCANTCAAAGC

Last Updated on 06.12.2018 16:11