Genotyping and primer details


Genotyping and primer details for SALK_052789c - At2g45760
Line ID SALK_052789c
Locus At2g45760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18847551 of line SALK_052789c Chr2:18847551
   
Insertion allele amplicon LBb3 - 45fp
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 45fp: 5'-GATCGACCACGTCATTATTCCTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18848202
   
wt amplicon (over InsPos) 09gs - 45fp
wt amplicon start Chr2:18847342
wt amplicon end Chr2:18848202
wt amplicon size 861 bp
Locus specific primer 45fp: 5'-GATCGACCACGTCATTATTCCTAT-3'
2nd locus specific primer 09gs: 5'-GGTTTCACCATGATAGACACATTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11