Genotyping and primer details


Genotyping and primer details for SALK_052963c - At3g22260
Line ID SALK_052963c
Locus At3g22260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7872107 of line SALK_052963c Chr3:7872107
   
Insertion allele amplicon LBb3 - 14ii
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 14ii: 5'-TGATCTTTGATCTGGGTTTCTGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7871267
   
wt amplicon (over InsPos) 10ir - 14ii
wt amplicon start Chr3:7871267
wt amplicon end Chr3:7872153
wt amplicon size 887 bp
Locus specific primer 14ii: 5'-TGATCTTTGATCTGGGTTTCTGTA-3'
2nd locus specific primer 10ir: 5'-TTTGAAGAGTCCACACAAGGTCTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11