Genotyping and primer details


Genotyping and primer details for SALK_053439c - At2g04025
Line ID SALK_053439c
Locus At2g04025 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:1277493 of line SALK_053439c Chr2:1277493
   
Insertion allele amplicon LBb3 - 69sa
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 69sa: 5'-TATTTGAATATGGACGTTATGGCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:1277944
   
wt amplicon (over InsPos) 07sx - 69sa
wt amplicon start Chr2:1276963
wt amplicon end Chr2:1277944
wt amplicon size 982 bp
Locus specific primer 69sa: 5'-TATTTGAATATGGACGTTATGGCA-3'
2nd locus specific primer 07sx: 5'-TCTTCTTCAACACTTTTGCTTGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11