Genotyping and primer details


Genotyping and primer details for SALK_053649c - At5g51690
Line ID SALK_053649c
Locus At5g51690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:21000973 of line SALK_053649c Chr5:21000973
   
Insertion allele amplicon LBb3 - 21md
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 21md: 5'-GTTCAATCATTTTGTGACCCTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21001546
   
wt amplicon (over InsPos) 68rd - 21md
wt amplicon start Chr5:21000470
wt amplicon end Chr5:21001546
wt amplicon size 1077 bp
Locus specific primer 21md: 5'-GTTCAATCATTTTGTGACCCTTTT-3'
2nd locus specific primer 68rd: 5'-CATTGACTCCATCAAATTCTCTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11